Fig. 2: Blood and hepatic lipid profile in WD + CCl4 treated mice.
A Serum levels of alanine aminotransferase (ALT), triglycerides (TG), and fatty acids (FA). B Hepatic TG and FA contents. C Relative mRNA expression levels of key genes involved in hepatic lipogenesis, including acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), sterol CoA-desaturase 1 (SCD1), and CD36 were measured using a real-time qPCR. D mRNA levels of peroxisome proliferator-activated receptor alpha (PPARα), peroxisome proliferator-activated receptor-gamma coactivator -alpha, (PGCα), carnitine palmitoyltransferase-1A (CPT1A), and CPT2 (n = 5–7 per group). E Representative hepatic hematoxylin and eosin (H&E)-stained and Oil red O-stained images. F Histological scores for hepatic steatosis, lobular inflammation, and hepatocyte ballooning based on H&E staining. G Quantification of Oil red O images. Results are expressed as the mean ± standard error of the mean (SEM). One-way ANOVA was used for multiple comparisons. #p < 0.05 vs. the control; ##p < 0.01 vs. the control; *p < 0.05 vs. the WD + CCl4 group; **p < 0.01 vs. the WD + CCl4 group.
