Fig. 3: LCA modulates angiocrine profile of LSECs thus initiating hepatocyte proliferation in vitro. | npj Gut and Liver

Fig. 3: LCA modulates angiocrine profile of LSECs thus initiating hepatocyte proliferation in vitro.

From: Lithocholic acid facilitates release of angiocrine factors by liver sinusoidal endothelial cells during liver regeneration

Fig. 3

Relative mRNA expression levels of (a) FXR & TGR5 (b) GATA4 & Id1 (c) CXCR4 & CXCR7 (d) Wnt2 & HGF as quantified by qRT-PCR. e Concentration of angiocrine factors Wnt2 & HGF in LCA-LSEC-CM using ELISA. f ELISA based concentration of cAMP in LCA-treated LSECs. g Representative immunofluorescence images of hepatocytes cultured for 16 h with PBS, DMSO, LCA 1 µM, LSEC-CM, LCA-LSEC-CM. The expression of PCNA (green) is shown and DAPI was used for nuclear staining (blue). Magnification ×40. h Graphical representation of PCNA+ cells cultured under different conditions. Scale bar 50 µm. i Representative immunofluorescence images of hepatocytes cultured for 16 h with LCA-LSEC-CM, before and after siRNA mediated silencing of Wnt2 in LSECs. j Graphical representation of PCNA+ hepatocytes before and after siRNA mediated silencing of Wnt2 in LSECs. k Relative mRNA expression levels of FoxM1B, Cyclin B1, Cyclin E, Cyclin A, Cyclin D1, FGFR4 as quantified by qRT-PCR of hepatocytes cultured with DMSO (hepatocyte specific media), DMSO-LSEC-CM, LCA-LSEC-CM. The data are normalized to Gapdh mRNA. Results are expressed as mean ± SD. (n = 5 each *p < 0.05 **p < 0.001, ***p < 0.0001).

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