Fig. 1: Whole body S100a11 knockout alleviates FFC diet-induced murine MASH.

A Schematic representation of the experimental regimen of WT and S100a11^−/− mice with 24 weeks of FFC diet feeding. A total of 6 CD-WT, 7 CD- S100a11^−/−, 5 FFC-WT, and 7 FFC- S100a11^−/− mice were included for downstream analysis unless otherwise specified. B Relative liver mass (liver to body mass percent) of the CD and the FFC diet fed WT and S100a11^−/− mice groups. C Plasma ALT levels in CD-fed and FFC-fed WT and S100a11^−/− mice. D Representative H&E stained liver samples from FFC-fed WT and S100a11^−/− mice. Scale bar: 50 μm. E Ten fields per H&E stained mouse liver samples were quantified for inflammatory foci at ×20 magnification. Each point represents the average value per mouse. F Steatosis was graded on H&E stained mouse liver samples per the NAFLD activity score at ×10 magnification. Each point represents the average value per mouse. G–I Lipid droplet morphometry was performed on H&E stained liver sections to calculate (G) total lipid droplet area, (H) average lipid droplet size, and (I) number of lipid droplets in CD and FFC-fed WT and S100a11^−/− mouse livers (n = 3 mice per group). J Representative images of picrosirius red stained liver sections are depicted along with quantification. Percent positive area of the picrosirius red stained positive collagen fibers was quantified using polarized microscopy: Scale bar: 50 μm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.