Fig. 5: Viral RNA detection in the tissues.

One cow was humanely euthanized on days 5, 6, and 7-post-infection for extensive tissue collection. a Viral loads in the mammary gland mucosa and body tissues were measured for the front right (FR, infected), rear left (RL, infected), front left (FL, non-infected), and rear right (RR, non-infected) quarters of cows #2 (blue), #3 (red), and #4 (black). b Viral RNA distribution in the respiratory tract of infected cows. Samples were titrated by qPCR, and viral loads were expressed as log10TCID50eq/g tissue. Samples with undetected virus titers were assigned the limit of detection value (LOD, 0.699 Log10 TCID50 equivalent/g tissue).