Extended Data Fig. 2: Characterization of a highly sensitive LFA for picomolar detection of sweat cortisol.
a, Effect of cortisol ab dilutions (1X, 2X, 4X, 6X and 8X) on the control/test line ratios at cortisol concentrations of 0, and 100 ng/mL. n = 3. b, Effect of flow rate on the control/test line ratios for cortisol concentrations of 0, and 100 ng/mL prior to working buffer optimization. n = 3. c, Effect of injection volumes (10, 15, and 20 μL) on the control/test line ratios at cortisol concentrations of 1 and 100 ng/mL. n = 3 prior to optimization of assay components. d, Effect of lighting color temperature (4500, 5000, 5500 K) on the control/test line ratios at cortisol concentrations of 0, 0.1, 1, 10, and 100 ng/mL. n = 3. e,f, Comparison of control/test line ratios of LFA images, at cortisol concentrations of 0, 0.1, 1, 10, and 100 ng/mL, taken with a smartphone camera (Iphone 16 pro) and a digital camera (Canon) at lighting color temperatures of e, 5500 K, and f, 4500 K. n = 3. Data are presented as mean values ± SD (n = 3 technical replicates).
