Extended Data Fig. 3: Analytical protocol for the test line and control line signals for wearable LFA.

a, Original image of an assay with color checker for calibration. Scale bar, 5 mm. b, Image of the assay after applying color calibration. Scale bar, 5 mm. c, Separated green channel image. Scale bar, 5 mm. d, Green channel image with a yellow line for LFA profile analysis. Scale bar, 5 mm. e, Straightened image of the yellow line for LFA profile analysis, representing the gray levels of the test and control lines. f, Gray level profiles of the straightened image of the yellow line showing the test and control line signals. g, Flow chart for LFA analysis.