Extended Data Fig. 4: Schematic and synthesis of the 1000 bp hairpin DNA. | Nature Sensors

Extended Data Fig. 4: Schematic and synthesis of the 1000 bp hairpin DNA.

From: Direct quantification of Piezo1 activation threshold through DNA-tethered extracellular force sensing

Extended Data Fig. 4: Schematic and synthesis of the 1000 bp hairpin DNA.The alternative text for this image may have been generated using AI.

The construct comprises five functional segments: a hairpin stem, a biotinylated region, a loop, a linker, and a thiol-modified end. The synthesis protocol involves four steps: (1) PCR amplification of the core hairpin sequence; (2) restriction enzyme digestion to generate cohesive ends; (3) annealing to form the hairpin structure; and (4) ligation to assemble the complete construct.

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