D-Amino acids are widely present in nature and display diverse physiological functions, however, their large-scale synthesis remains challenging for specific amino acids. Here, the authors design hyper-thermostable ancestral L-amino acid oxidases (HTAncLAAO2), catalyzing the chemoenzymatic synthesis of D-tryptophan from L-tryptophan at a preparative scale.
- Yui Kawamura
- Chiharu Ishida
- Shogo Nakano
