Nanofactories are artificial vesicles that shields the internal enzymes while allowing small substrates to permeate the membranes for catalytic reactions. A key challenge in nanofactories is achieving efficient enzyme encapsulation. The constructed self-assembled vesicles from a thermoresponsive peptoid-based block copolymer achieved a high enzyme encapsulation efficiency of more than 50%. It is hypothesized that this high performance stems from a temperature-induced coacervate-to-vesicle phase transition. The resulting enzyme-loaded vesicles acted as robust nanoreactors, protecting enzymes from external proteases, while exhibiting selective permeability of small molecules on the basis of physicochemical properties.
- Yota Okuno
- Tomoki Nishimura
- Kazunari Akiyoshi
