Search

RNA-seq is widely used for transcriptome analysis. Here, the authors analyse a wide spectrum of RNA-seq workflows and present a comprehensive analysis protocol named RNACocktail as well as a computational pipeline leveraging the widely used tools for accurate RNA-seq analysis.

A tunnel field-effect transistor with spin-dependent outputs that are voltage controllable and reversible can be created using a dual-gated graphene/CrI₃/graphene tunnel junction.

Monolayer graphene has no electronic band gap. Bilayer graphene does, and can be controlled by an electric field. And for trilayer graphene, infrared transmission measurements indicate both situations are possible depending on the stacking of the layers.

Tunnelling spectroscopy reveals a continuous closing of the superconducting gap at low temperature and high in-plane magnetic field in few-layer NbSe₂, due to the Ising spin–orbit coupling of these materials.

Gene isoforms are quantified with a machine learning method that optimally integrates long and short sequencing reads.

The valley Hall effect in bilayer MoS₂ transistors can be controlled using a gate voltage and the induced valley polarization imaged with Kerr microscopy.

The Haldane model is a paradigmatic example of topological behaviour but has not previously been implemented in condensed-matter experiments. Now a moiré bilayer is shown to realize this model with the accompanying quantized transport response.

A Kondo lattice was realized in AB-stacked MoTe₂/WSe₂ moiré bilayers and widely and continuously gate-tunable Kondo temperatures were demonstrated.

Here the authors apply long read sequencing to identify transcripts expressed during early zebrafish embryogenesis, including transposable elements (TE). They find zygotic TE activation occurs later than zygotic genome activation (ZGA) and has a bias for nuclear localized TE transcripts.

Electrostatic doping in vertical van der Waals CrI₃–graphene heterostructures provides means to control the magnetic properties of monolayer and bilayer CrI₃.

Sperm carry diverse RNAs to the zygote. Here the authors employ single molecule long-read sequencing to distinguish intact RNAs from fragmented RNAs, reporting thousands of intact sperm RNA species conserved in mouse and human sperms.

A two-fold rotational symmetry is observed in the superconducting state of NbSe₂. This is strikingly different from the three-fold symmetry of the lattice, and suggests that a mixed conventional and unconventional order parameter exists in this material.

The superconducting properties of NbSe₂ as it approaches the monolayer limit are investigated by means of magnetotransport measurements, uncovering evidence of spin–momentum locking.

Enhanced electron–phonon interactions in mono- and few-layer NbSe₂ result in a significantly increased transition temperature of charge density waves compared with values in the bulk.

The appealing electronic properties of the monolayer semiconductor molybdenum disulphide make it a candidate material for electronic devices. The observation of tightly bound trions in this system—which have no analogue in conventional semiconductors—opens up possibilities for controlling these quasiparticles in future optoelectronic applications.

Valley magnetization in single-layer MoS₂ is demonstrated by breaking the three-fold rotational symmetry via uniaxial stress. The results are consistent with a theoretical model of valley magnetoelectricity driven by Berry curvature effects.

Structural and biophysical analyses reveal that Helicobacter pylori CtpA forms a hexamer with asymmetric activation, enabling processive and adaptor-independent proteolysis through coordinated inter-subunit interactions.

This Registered Report presents the results of the Long-read RNA-Seq Genome Annotation Assessment Project, which is a community effort for benchmarking long-read methods for transcriptome analyses, including transcript isoform detection, quantification and de novo transcript detection.

Study of WSe₂/WS₂ moiré superlattices reveals the phase diagram of the triangular-lattice Hubbard model, including a Mott insulating state at half-filling and a possible magnetic quantum phase transition near 0.6 filling.

Renee Reijo Pera, Vittorio Sebastiano and colleagues identify three human pluripotency-associated transcripts (HPATs) that function in preimplantation development. They find that these three HPATs are also required for efficient nuclear reprogramming and that one, HPAT5, interacts with let-7 to modulate gene expression during reprogramming and differentiation.

Optical anisotropy and electronic compressibility measurements are used to uncover stripe phases, where the rotational symmetry of charge density is spontaneously broken, in a two-dimensional semiconductor moiré superlattice.

A coupling of mechanical vibrations and magnetism and strain-tuning of the exchange interactions, is demonstrated for few-layer CrI₃.

Pressure-induced changes in the magnetic order of atomically thin van der Waals crystals are revealed and attributed to changes in the stacking arrangement.

Electrons in two-dimensional semiconductor moiré materials experience competing magnetic interactions. Magneto-optical measurements of moiré devices with controlled screening of the Coulomb interactions now evidence a Wigner–Mott insulating state with frustrated magnetic interactions.

The realization of strongly correlated bosons in a solid-state lattice is challenging. Here, the authors trap interlayer excitons in an angle-aligned WS₂/bilayer WSe₂/WS₂ multilayer moiré lattice and observe correlated insulating states.

Here the authors apply low-input methyl RNA immunoprecipitation and sequencing to map the N⁶-methyladenosine landscape during mouse oocyte and early embryo development. They show that RNAs derived from retrotransposons are often N⁶-methyladenosine marked and so are many genes important for the maternal-to-zygotic transition.

A picogram-scale m⁶A mapping method is applied to single mouse oocytes and embryos in vivo.

A preclinical murine asthma model shows that exposure to air pollution particulates during development causes worsened airway hyperreactivity and points to a transcriptomic and DNA methylation memory of developmental environment insult in adult offspring.

Au and colleagues outline the field of nanopore sequencing.