A reduction-responsive oligonucleotide was successfully constructed by post-modification of an oligonucleotide with a diazo compound bearing a 4-nitrobenzyl group as a reduction-responsive cleavable moiety. High-performance liquid chromatography and mass spectrometry were used to reveal the introduction of the 4-nitrobenzyl group to the 5′-phosphate group of the oligonucleotide, and the subsequent reduction-triggered recovery of the original oligonucleotide. The protocol used for the preparation of this reduction-responsive oligonucleotide is simple and it will have various applications in the fields of chemical and synthetic biology.
- Nanami Shirakami
- Sayuri L. Higashi
- Masato Ikeda
