Transcription factor p53 is sensitive and selective to a broad variety of cellular environments, with oxidative stress demonstrated to weaken p53-DNA binding for certain promoters, but the precise mechanisms by which the physiologically relevant DNA-p53 tetramer complex senses cellular stresses caused by H2O2 remain unknown. Here, native mass spectrometry and ion mobility–mass spectrometry are coupled with chemical labelling and H2O2-induced oxidation to probe the mechanism of redox regulation of the p53-p21 complex.
- Manuel David Peris-Díaz
- Artur Krężel
- Perdita Barran