The NSUN family, comprising seven members, is the primary RNA m5C methyltransferase in higher eukaryotes. Here, the authors demonstrate that NSUN7 is a catalytically inactive RNA m5C protein due to sequence variations in its motif IV. Instead, the authors reveal a noncatalytic, regulatory role of NSUN7 in sperm flagellum assembly.
- Jing Li
- Wen-Yu Zhu
- Ru-Juan Liu
