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CRISPR-Cas12a diagnostics are limited by strict PAM requirements. Here, authors show that higher temperatures activate numerous noncanonical PAMs, enabling a versatile one-pot platform that improves sensitivity, specificity, and rapid on-site pathogen detection.

Engineered mucus-tethering bispecific nanobodies neutralize and entrap viruses to enhance mucosal immunity, preventing influenza infection and limiting SARS-CoV-2 transmission.

This study shows that the wheat major QTL gene-encoded sugar metabolic enzyme HtL1/FBA10 undergoes vernalization-induced dynamic changes in O-GlcNAcylation and phosphorylation, enhancing its enzymatic activity and increasing VRN1 gene expression for flowering.

Isotropic tissue magnification is integrated with matrix-assisted laser desorption/ionization mass spectrometry imaging to enable untargeted spatial proteomics at micrometre resolution and with high protein identification rates in multiple tissue types.

A new artificial intelligence model, DeepSeek-R1, is introduced, demonstrating that the reasoning abilities of large language models can be incentivized through pure reinforcement learning, removing the need for human-annotated demonstrations.

Ultrapure water is usually used as a feedstock for proton exchange membrane (PEM) electrolysers because trace contaminants can cause failure. Here the authors show that PEM electrolysers can run on impure tap water when Brønsted acid oxides are used at the cathode to create a strongly acidic microenvironment.

Use of ether-based electrolytes in Na-ion batteries is challenged with oxidation at high voltages. Here, authors report passivation of vulnerable ether solvents, enabling cathode electrolyte interphase enriched with NaF and NaN_xO_y and elevating the oxidation resistance of the electrolyte to 4.8 V.

Rational design of gas diffusion layers (GDL) is critical for enhancing the performance of proton exchange membrane fuel cells. Here, the authors employ Bayesian machine learning to design aligned GDLs, achieving a limiting current density of 7200 mA cm^-2.

Autophagy prolongs the survival of cells in stressful conditions but its role in cancer is unclear. Here, Rao et al. show that loss of the autophagic protein Atg5 enhanced cancer incidence but impaired tumour progression in a mouse model of lung cancer.

This study shows that global warming has led to both earlier and later snowmelt floods in different regions over the past 70 years, challenging the prevailing idea that warming always advances floods and highlighting the complex nature of snowmelt flood timing shifts.

LC-MS-based proteomics often relies on data-dependent acquisition (DDA) for quality control. Here, the authors demonstrate that data-independent acquisition (DIA) outperforms DDA in detecting subtle changes in LC-MS status in large-scale quantitative proteomics experiments. They further prioritized 15 QC metrics and developed an AI model, implemented in a free software called iDIA-QC, for detecting LC-MS faults.

Selective CO₂-to-liquid conversion remains a major challenge due to inertness of CO₂. This work designs Pd nanoneedles with bent Pd–Pd bonds, enabling >99% selective formic acid production under mild conditions by enhancing CO₂ activation.

CRISPR systems are powerful tools for gene editing and diagnostics, but their regulation is challenging. Here, the authors present DNACas, a light-controlled method using photocleavable phosphorothioate DNA to modulate CRISPR activity, enabling precise gene editing and one-pot diagnostic detection.

Cohort-scale integration of clinical and proteomic data from tumor tissues in patients with pancreatic ductal adenocarcinoma led to the identification of a prognostic risk model for patient stratification as well as biomarkers of response to adjuvant chemotherapy, validated in independent external cohorts.

Gong et al. uncover a role for the Golgi network in ER-associated endosome fission, showing that a Golgi-derived SEC14L2 compartment mediates ER-associated endosome fission by promoting PtdIns4P to PtdIns3P conversion.

Biorenewable feedstocks are important for developing more sustainable materials, but developing processes can be challenging due to incompatible reaction conditions. Here, the authors report the one-pot preparation of aggregation-induced emission luminogens from lignin moieties.

Population level transcriptomics measurements miss bacterial heterogeneity. Here the authors report smRandom-seq, a droplet-based high-throughput single-microbe RNA-seq assay, using random primers for in situ cDNA generation, droplets for single-microbe barcoding, and CRISPR-based rRNA depletion.

Membrane contact sites between organelles have been shown to play important biological roles. Here, the authors show that at the ER, PDZD8 associates with Protrudin and also with Rab7 endosomes and recruits mitochondria to form three-way contacts.

Synthetically replicating transmembrane protein signal transduction is a gaol of synthetic biology. Here, the authors show how the dimerization of synthetic transmembrane DNA receptors can be used to engineer sensing and actuation cascades in response to external molecular signals.

Analysis of streamflow measurements from 1950 to 2020 across 3,049 snow-affected catchments over the Northern Hemisphere shows that seasonal streamflow occurs earlier in snow-heavy catchments but later in less snowy regions.

Direct seawater electrolysis is promising for sustainable hydrogen production but suffers severe side reactions and corrosion. Here, the authors report a corrosion-resistant electrocatalyst with in situ-formed chloride-ion-repelling cation layer for efficient and long-lasting seawater oxidation.

Stable manipulation of polar skyrmions is challenging because of the underlying competitive energy scales. Now thermal excitation has been demonstrated to be an effective way to control such topological states.

Tang, Yue, Xu and colleagues map the proteome of several thousand individuals over a 9-year period to identify potential biomarkers of ageing and of age-associated diseases.

This study explores the performance of deep-learning models for super-resolution imaging and introduces models that utilize frequency content information in the Fourier domain to improve SIM reconstruction under low-SNR conditions.

Yu and colleagues present a single-cell transcriptomic landscape of olfactory neuroblastoma, characterizing different tumor subtypes and cancer cell responses to drug treatments.

A multistate analysis of 341,159 adults from the UK Biobank shows that the development and mortality of cardiometabolic multimorbidity are associated with biological aging, as measured by the Klemera–Doubal Method Biological Age and PhenoAge algorithms.

Xu, Liang, Li, Dang et al. delineate the clathrin-associated fast endosomal recycling pathway, which involves clathrin-associated carriers derived from early endosomes partially fusing with the plasma membrane before release from the membrane.

The assembly of eight high-quality rapeseed genomes allows identification of presence and absence variations (PAVs) and small variations. PAV-based genome-wide association analysis uncovered causal variations for agronomic traits and ecotype differentiation.

Microwell-seq is used to generate single-cell, whole-body expression landscapes of zebrafish, Drosophila and earthworm. A deep-learning model, Nvwa, predicts gene expression from DNA sequence and identifies regulatory programs shared across eight species.

The Mexican axolotl is a well-established tetrapod model for regeneration and development. Here the authors report a scRNA-seq method to profile neotenic, metamorphic and limb development stages, highlighting unique perturbation patterns of cell type-related gene expression throughout metamorphosis.

Transmembrane channels have inspired the development of biomimetic channels. Here, the authors present a class of artificial nanochannels based on DNAzyme-functionalized glass nanopipettes to allow for the control of channel permeability.

α-ketoglutarate is an intermediate of the Krebs Cycle that was recently reported to extend lifespan in C.Elegans. Here, the authors show that administration of α-ketoglutarate to mice reduces age-related bone loss, by ameliorating senescence of bone-marrow derived mesenchymal stem cells.

Integrating genetic information with circulating proteomics can help understand mechanisms of disease. Here, the authors conduct genome-wide association analyses of the serum proteome in 2,958 Han Chinese individuals, uncovering proteins which may contribute to ancestry differences in cardiometabolic disease susceptibility.

A study presents the results of surveillance for SARS-CoV-2 in animal and environmental samples from a market linked to early human cases of coronavirus disease 2019.

Rationalized deep learning improves image reconstruction by incorporating prior knowledge of illumination patterns.

Rev-erbα is a known regulator of the circadian clock. Here, the authors show that Rev-erbα is also a regulator of synaptic dysfunction in preclinical models of epilepsy.

Quantifying rapidly progressing three-dimensional forces generated by cells remains a major challenge in mechanobiology. Here, the authors show that combining traction force microscopy with astigmatic imaging permits sensitive out-of-plane force estimation on the second timescale.

The methylammonium lead halide perovskites have shown excellent optoelectronic properties but the field-effect transistors are much less studied. Here Yu et al. synthesize micrometer-thin crystals of perovskites with low surface contamination and make ambipolar transistor devices with high mobilities.