Extended Data Fig. 5: Age-dependent increases in N-glycan branching suppress T cell activity in human females.
a-d) Human PBMCs from healthy females (a, c) or males (b, d) as indicated were analyzed for L-PHA binding by flow cytometry gating on CD8+ T cells (a, b) or CD19+ B cells (c, d). e, f) CD4+ TN cells (CD45RA+CD45RO−) from healthy females (e) and males (f) under the age of 65 were analyzed for L-PHA binding by flow cytometry. g) Human PBMCs from young (22-38 years old) and old (90-94 years old) female subjects were analyzed for L-PHA binding on CD4+ TN cells (CD45RA+CD45RO−) before or after 96 hours of culture in complete media. Shown is the ratio or each old subject over the average of the young at the two timepoints. Each symbol represents a single individual. R2 and p-values by linear regression (a-f) or by paired one-tailed t test, following passage of Shapiro–Wilk normality test (g). Error bars indicate mean ± s.e.m.
