Extended Data Fig. 7: Integrin β3 reduction in aortic SMCs attenuates phosphorylation of AKT and ERK and PDGF-B-induced dorsal ruffles.
a,b, Aortic lysates of wild type (WT) or Itgb3(−/−) mice were subjected to Western blot for integrin β3, GAPDH and phosphorylated (p-) and total AKT (a) with densitometry of each protein relative to GAPDH and normalized to WT (b). n = 6 mice per genotype. c–f, Mouse aortic SMCs were isolated and treated with Itgb3 siRNA or scrambled (Scr) RNA. Cell lysates were subjected to Western blot for integrin β3, GAPDH as well as phosphorylated and total AKT, PAK1 and ERK (c) with densitometry of each protein relative to GAPDH and normalized to Scr (d). In e, following siRNA treatment, SMCs were serum starved overnight, treated with PDGF-B (10 ng/mL) for the indicated times and stained with FITC-phalloidin. Arrowheads indicate dorsal ruffles with boxed regions shown as close-ups below. Scale bar, 25 μm. Quantification of dorsal ruffles is shown in (f). n = 6 experiments. All data are means ± SD, and two-tailed Student’s t-test was used.
