Extended Data Fig. 2: Supplementary data related to Fig. 4.
a, Representative images of ovastacin in young, aged, and Spd+aged M II oocytes. Scale bar, 30 μm. b, The fluorescence intensity of ovastacin signals was measured in young (n = 29), aged (n = 25), and Spd+aged (n = 25) M II oocytes. ****P < 0.0001, ****P < 0.0001. Data were expressed as mean value (mean ± SD) of at least three independent experiments. Statistical significance was determined by two-tailed unpaired t-test. c, Co-staining of mitochondrion and spindle in young MII oocytes. Scale bars, 40 μm, 20 μm.
