Fig. 7: Aging-associated accumulation of senescent-like MuSCs and progenitors within muscle injury zones in spatial transcriptomic maps.
a–d, Spatial transcriptomic maps of young (a and b) and geriatric (c and d) TA muscles 5 days after injury. Beads are colored by cell type (a and c) and injury zone (b and d). Scale bars, 500 µm. e,f, Percentage of cells of select cell types in the injury zone (e) and the noninjury zone (f) in the young and geriatric samples. g–n, Zoomed-in section of the young (g, i, k and m) and geriatric (h, j, l and n) spatial maps (as outlined in a–d) with beads colored by all cell types (g and h), MuSCs and progenitors (i and j), fusing myocytes (k and l) and one-way FBR Sen Score (m and n). o, Box plots of the one-way FBR Sen Score in the MuSCs and progenitors and all other cell types within the full transcriptomic map and within the injury and noninjury zones. The center line is the median, box limits are the upper and lower quartiles, whiskers are the 1.5 times the interquartile range and diamonds are the outliers. From left to right, n = 168, 99, 120, 88, 48, 11, 31,936, 2,2821, 11,361, 9,609, 20,575 and 13,212. Significance was evaluated using a two-sided Mann–Whitney U test.
