Extended Data Fig. 5: Confirmation of astrocyte substate transitions in vivo, related to Fig. 3.
From: Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration
a, Heatmap displaying the expression patterns of neuroprotective and neurotoxic substate markers in purified brain cortical astrocytes from a mouse model injected intraperitoneally with PBS or LPS at 3- and 24-hours post-injection, n = 4 mice for saline-3h and LPS-24h groups, n = 5 mice for saline-24h and LPS-3h groups. b, Heatmap showing the expression trends of neuroprotective and neurotoxic substate markers in the whole cortical tissues of mice injected with LPS over time, n = 3 mice per group. c,d, QPCR analysis for the expression of neurotoxic (c) and neuroprotective (d) substate markers in sorted TNFRSF12A-positive astrocytes, purified from LPS-injected mice, after a 36-hour ex vivo culture in serum-free medium, n = 3 replicates per group. TNFRSF12A-negative astrocytes purified from PBS-injected mice served as control. For each marker, the mRNA expression level was normalized to that in one of the control groups. In a and b, one-way ANOVA tests were used. For each gene, the expression level was compared to that in the saline 3 h group (a) or LPS 0 h group (b), *P < 0.05 (an asterisk denotes significance for the average of all replicates). The data in c and d were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Unpaired two-tailed Student’s t-tests were used for statistical analyses; the exact P values are provided in the Source data.
