Extended Data Fig. 7: Effects of mTOR inhibitor and agonist on the expression of astrocyte substate markers, related to Fig. 5.
From: Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration
a, Analysis of the enrichment scores for mTOR signaling pathways in astrocytes isolated from WT and APP/PS1 mice with different ages26. The differentially expressed genes, which are the same to the genes used in Fig. 5d, in the mTOR signaling pathways were used to analyze the enrichment scores, n = 3 mice per group. b-d, Immunoblotting analysis showing the phosphorylation and expression trends of mTOR, S6, and 4EBP1 over time following IL-1α (b), TNF (c), or C1q (d) stimulation. e-h, QPCR results for the expression levels of neurotoxic substate markers of H2-T23 (e), H2-D1 (f), Gbp2 (g), and C3 (h) in astrocytes treated with saline, mTOR activator MHY1485, ITC, or the combination of MHY1485 and ITC. In e-h, data were presented as mean ± SEM, n = 3 replicates per group. *P < 0.05, **P < 0.01, ***P < 0.001. One-way ANOVA and Tukey’s multiple comparisons test were used for each time point; the exact P values are provided in the Source data.
