Extended Data Fig. 1: Evaluation of the stability of neurotoxic and neuroprotective substates in response to inflammatory stimuli, related to Fig. 1.
From: Modulating mTOR-dependent astrocyte substate transitions to alleviate neurodegeneration
a-c, Relative mRNA expression levels of pan-reactive (a), neurotoxic (b), and neuroprotective (c) substate markers in MD-astrocytes treated with PBS or ITC for 7 days. d, Heatmap depicting the expression patterns of pan-reactive astrocyte markers, neurotoxic substate markers, and neuroprotective substate markers in astrocytes treated with ITC for 6 h, followed by ITC withdrawal to 48 h. e, Heatmap depicting the expression patterns of pan-reactive astrocyte markers, neurotoxic substate markers, and neuroprotective substate markers in astrocytes treated with ITC for 2 h, followed by ITC withdrawal to 48 h. All data were presented as mean ± SEM, with n = 3 replicates per group. *P < 0.05, **P < 0.01, ***P < 0.001. In a-c, unpaired two-tailed Student’s t-tests were used. In d and e, one-way ANOVA tests were used. For each gene, the expression level was compared to that in the ITC 0 h group (*P < 0.05, an asterisk denotes significance for the average of all three replicates). The exact P values are provided in the Source data.
