Extended Data Fig. 1: The absence of Sema4A leads to excessive myeloid expansion and premature hematopoietic aging-like phenotype. | Nature Aging

Extended Data Fig. 1: The absence of Sema4A leads to excessive myeloid expansion and premature hematopoietic aging-like phenotype.

From: Niche-derived Semaphorin 4A safeguards functional identity of myeloid-biased hematopoietic stem cells

Extended Data Fig. 1: The absence of Sema4A leads to excessive myeloid expansion and premature hematopoietic aging-like phenotype.

A. Representative images of H&E staining of femurs from aged WT/Sema4AKO mice (n = 4 mice per group). B. Bone marrow cellularity of aged WT/Sema4AKO mice (n = 4 mice per group). C. Gating strategy for flow cytometric analysis of primitive bone marrow subsets (WT aged mice are shown). LT-HSC denotes long-term HSC. D. Gating strategy for flow cytometric identification of myHSC and lyHSC based on intensity of CD150 expression. E. Absolute numbers of myHSC and lyHSC in young and aged WT mice (n = 6 mice for young group and n = 4 mice for aged group) F. Representative plots of myHSC quantification in aged WT/Sema4KO mice. G. Absolute number of myHSC and lyHSC in individual aged WT/Sema4AKO mice. P values are shown. Statistical significance was assessed by two-tailed t-test. Mean +/- (SEM) are shown.

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