Extended Data Fig. 6: OPCs upregulate C4b, Hif1a, and Ctnnb1 mRNA with aging.
From: Transcriptional profiles of mouse oligodendrocyte precursor cells across the lifespan
a. FISH with immunofluorescence staining (IF) for Pdgfra and C4b in P35 and P315 Matn4-mEGFP mouse cortex. OPC cell body masks were created based on EGFP fluorescence and Pdgfra FISH signal and used to quantify C4b transcript puncta/OPC (Two-tailed, Mann-Whitney U test, n = 65, 49 cells, * p-value = 0.0177, error bars = SEM). b. FISH with IF staining for Pdgfra and Hif1a in the P35 and P315 Matn4-mEGFP mouse cortex. Hif1a transcript puncta/OPC was quantified as described above (Two-tailed, Mann-Whitney U test, n = 56, 71 cells, * p-value = 0.0385, error bars = SEM). c. FISH with IF staining for Pdgfra and Ctnnb1 in the P35 and P315 Matn4-mEGFP mouse cortex (Two-tailed, Mann-Whitney U test, n = 59, 55 cells, ** p-value = 0.0069, error bars = SEM). The exact p-values are reported in Source Data file for Extended Data Fig. 6.
