Extended Data Fig. 1: Matn4-mEGFP expression is restricted to OPCs and a subset of neurons.
From: Transcriptional profiles of mouse oligodendrocyte precursor cells across the lifespan
a. Matn4 expression is specific to OPCs and newly-formed oligodendrocytes (NFO) in 6-7 week-old mouse V1 cortex (reanalysis of a publicly available scRNA-seq dataset18). b. Genotyping result of Matn4-mEGFP mouse line. Wildtype (wt) band size is 178 bp whereas the mutant, knock-in band size is 356 bp. c. EGFP signal in the optic nerve of Matn4-mEGFP mouse line is restricted to NG2 + PDGFRα+ OPCs. d. Matn4-mEGFP is also expressed by hippocampal granule cells and neurons in the somatosensory cortex barrel field and retrosplenial cortex. e. Matn4-mEGFP signal is absent from Iba1+ microglia and GFAP+ astrocytes. f. In vivo imaging of GFP+ cells in Matn4-mEGFP, NG2-mEGFP, and Pdgfra-CreER; RCE mouse lines. None of the vascular cells (red arrowheads) express EGFP in the cortex of Matn4-mEGFP mice.
