Extended Data Fig. 2: Validation of Cell Identity after IR Treatment.
From: The secretome of senescent monocytes predicts age-related clinical outcomes in humans
a, Classical SA Beta-gal staining performed 7 days after IR treatment showed increased number of beta galactosidase positive cells as indicated by the formation of blue color in senescent cells as compared to the proliferating controls. b, Brightfield microscopy images to assess any change in morphology after senescence induction shows conservation of suspension cell characteristic of monocytes 7 days after IR treatment. Flow cytometry analysis performed to confirm cell identity after IR treatment shows no increase the monocyte differentiation markers c, CD14 and d, CD11b indicating conservation of monocyte cell lineage after IR treatment.
