Extended Data Fig. 2: scRNA-seq analysis of senescence gene expression.

a, UMAPs of p16-tdTomato mRNA expression in ND, HFD, and HFD + ABT-737 treated mice. b, UMAP plot of Cdkn2a mRNA expression across all clusters and conditions (left). Violin plot of Cdkn2a mRNA expression across all clusters (right). c, UMAP plot of Cdkn1a mRNA expression across all clusters and conditions (left). Violin plot of Cdkn1a mRNA expression across all clusters (right). d, GSEA SenMayo plots comparing HFD vs ND and HFD + ABT-737 vs HFD for Clusters 15, 20, 21, 3, 16, and 27. e, GSEA CellAge plots comparing HFD vs ND and HFD + ABT-737 vs HFD for Clusters 6, 17, 7, and 11.