Extended Data Fig. 7: Doxorubicin-induced cellular senescence in wild type mice. | Nature Aging

Extended Data Fig. 7: Doxorubicin-induced cellular senescence in wild type mice.

From: Single-cell and spatial transcriptomics map senescent vascular cells in arterial remodeling during atherosclerosis in mice

Extended Data Fig. 7: Doxorubicin-induced cellular senescence in wild type mice.The alternative text for this image may have been generated using AI.

a, RT-qPCR analysis of the levels of Cdkn2a and Lmnb1 mRNAs in aortic roots of mice treated with vehicle, doxorubicin, and doxorubicin followed by ABT-737. b, RT-qPCR analysis of the levels of Ltbp2, Sparc, Mgp, Lum, and Cd9 mRNAs in aortic root tissue as described (a). c, RT-qPCR analysis of the levels of Apoe, Lgals3, Cst3, Tnfrsf11b, and Prg4 mRNAs in aortic root tissue as described (a). d, RT-qPCR analysis of the levels of Mmp3, Ctss, Lcp1, and Comp mRNAs in aortic root tissue as described (a). In a-d, data represent the means ± SD for DMSO, n = 4; Doxo, n = 4; Doxo + ABT-737, n = 3 (females), and DMSO, n = 4; Doxo, n = 4; Doxo + ABT-737, n = 4 (males). n represents biological replicates. In panel (a), Cdkn2a, Doxo vs. DMSO, p = 0.0162 (females) and p = 0.002 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0226 (females) and p = 0.0034 (males); Lmnb1, Doxo vs. DMSO, p = 0.5863 (females) and p = 0.1807 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.1013 (females) and p = 0. 3449 (males). In panel (b), Ltbp2, Doxo vs. DMSO, p = 0.0004 (females) and p ≤ 0.0001 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0007 (females) and p ≤ 0.0001 (males); Sparc, Doxo vs. DMSO, p ≤ 0.0001 (females) and p ≤ 0.0001 (males), whereas Doxo + ABT-737 vs. Doxo, p ≤ 0.0001 (females) and p = 0.0002 (males); Mgp, Doxo vs. DMSO, p = 0.9928 (females) and p = 0.9928 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.7181 (females) and p > 0.9999 (males); Lum, Doxo vs. DMSO, p = 0.0018 (females) and p = 0. 0002 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0018 (females) and p = 0.001 (in males); Cd9, Doxo vs. DMSO, p = 0.0014 (females) and p = 0. 0011 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0031 (females) and p = 0.0019 (males). In panel (c), Apoe, Doxo vs. DMSO, p = 0.0194 (females) and p = 0. 3607 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.5746 (females) and p = 0.4692 (males); Lgals3, Doxo vs. DMSO, p ≤ 0.0001 (females) and p ≤ 0.0001 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0002 (females) and p ≤ 0.0001 (males); Cst3, Doxo vs. DMSO, p = 0.0008 (females) and p ≤ 0.0001 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0021 (females) and p ≤ 0.0001 (males); Tnfrsf11b, Doxo vs. DMSO, p = 0.3051 (females) and p = 0.9339 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.1425 (females) and p = 0.9833 (males); Prg4, Doxo vs. DMSO, p ≤ 0.0001 (females) and p = 0.0177 (males), whereas Doxo + ABT-737 vs. Doxo, p ≤ 0.0001 (females) and p = 0.0283 (males). In panel (d), Mmp3, Doxo vs. DMSO, p ≤ 0.0001 (females) and p ≤ 0.0001 (males), whereas Doxo + ABT-737 vs. Doxo, p ≤ 0.0001 (females) and p ≤ 0.0001 (males); Ctss, Doxo vs. DMSO, p = 0.0007 (females) and p = 0.0025 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0017 (females) and p = 0.005 (males); Lcp1, Doxo vs. DMSO, p ≤ 0.0001 (females) and p ≤ 0.0001 (males), whereas Doxo + ABT-737 vs. Doxo, p ≤ 0.0001 (females) and p ≤ 0.0001 (males); Comp, Doxo vs. DMSO, p = 0.0005 (females) and p = 0.0029 (males), whereas Doxo + ABT-737 vs. Doxo, p = 0.0027 (females) and p = 0.004 (males). Significance was established using One-Way Anova for each sex, *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001.

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