Extended Data Fig. 1: Atherosclerotic plaque pathology analysis and quality control analysis. | Nature Aging

Extended Data Fig. 1: Atherosclerotic plaque pathology analysis and quality control analysis.

From: Single-cell and spatial transcriptomics map senescent vascular cells in arterial remodeling during atherosclerosis in mice

Extended Data Fig. 1: Atherosclerotic plaque pathology analysis and quality control analysis.The alternative text for this image may have been generated using AI.

a, Quantification of plaque size presented by sex and condition (ND, HFD, and HFD + ABT-737). For female mice, ND (n = 4), HFD (n = 3), and HFD + ABT-737 (n = 2) and HFD vs. ND, p = 0.0006 and HFD + ABT-737 vs. HFD, p = 0.576, whereas for male mice, ND (n = 4), HFD (n = 2), and HFD + ABT-737 (n = 3) and HFD vs. ND, p = 0.0004 and HFD + ABT-737 vs. HFD, p = 0.8876. b, Quantification of necrotic core area presented by sex and condition (ND, HFD, and HFD + ABT-737). For female mice, HFD (n = 3) and HFD + ABT-737 (n = 2) HFD + ABT-737 vs. HFD, p = 0.1929, whereas for male mice, HFD (n = 2) and HFD + ABT-737 (n = 2) and HFD + ABT-737 vs. HFD, p = 0.4985. c, Quantification by plaque fibrous cap thickness presented by sex and condition (ND, HFD, and HFD + ABT-737). For female mice, HFD (n = 3) and HFD + ABT-737 (n = 2) and HFD + ABT-737 vs. HFD, p = 0.2033, whereas for male mice, HFD (n = 2) and HFD + ABT-737 (n = 2) and HFD + ABT-737 vs. HFD, p = 0.4552. d, Representative H&E staining of aortic roots from ND, HFD, and HFD + ABT-737. The scale bar is 200 µm. e, Quantification of collagen fibers (Aniline blue) area / area of aortic root presented by sex and condition (ND, HFD, and HFD + ABT-737). For female mice, HFD (n = 2) and HFD + ABT-737 (n = 2) HFD + ABT-737 vs. HFD, p = 0.2467, whereas for male mice, HFD (n = 2) and HFD + ABT-737 (n = 2) and HFD + ABT-737 vs. HFD, p = 0.7036. f, Representative senescence-associated beta galactosidase (SA-β-gal) activity staining of aortic roots from ND, HFD, and HFD + ABT-737. The scale bar is 200 µm. g, Quantification of SA-β-gal (ND, HFD, and HFD + ABT-737). ND (n = 5), HFD (n = 5) and HFD + ABT-737 (n = 5) and for significance, HFD vs. ND, p ≤ 0.0001 and HFD + ABT-737 vs. HFD, p = 0.069. h, Pulse wave velocity (PWV) measurements presented by sex and condition (ND, HFD, and HFD + ABT-737). For female mice, ND (n = 3), HFD (n = 2), and HFD + ABT-737 (n = 4) and HFD vs. ND, p = 0.748 and HFD + ABT-737 vs. HFD, p = 0.9597, whereas for male mice, ND (n = 3), HFD (n = 2), and HFD + ABT-737 (n = 2) and HFD vs. ND, p = 0.0294 and HFD + ABT-737 vs. HFD, p = 0.1001. i, Quality control table of the scRNA-seq samples including estimated number of cells, fraction reads in cells, mean reads per cell, median UMI counts per cell, median genes per cell, and total genes detected. j, Heatmap of normalized expression levels of classical senescence associated mRNAs either enriched in HFD and reduced by ABT-737 treatment, or conversely, reduced by HFD and increased by ABT-737 treatment in mice (Lmnb1 and Lbr mRNAs). Significance was established using Two-Way ANOVA (for data in graphs a-c, e, and h) and One-Way ANOVA (for data in graph g) with multiple comparisons. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001; ****, p ≤ 0.0001.

Source data

Back to article page