Extended Data Fig. 5: MFE-2 deficiency drove dysregulated lipid β-oxidation and exacerbated microglial activation in response to Aβ.
a. Untargeted metabolism profile in FACS sorted microglia from 8-month-old ΔMFE-2 and Flox mice by LC-MS. Differential metabolites were identified using a two-sided Student’s t-test. (n = 4 in Flox group and n = 6 in MFE-2 knockout group. Microglia from three brains were pooled for each sample, male.) b. The cultured primary microglia from P0 Flox and ΔMFE-2 mice were input for FFAs quantitative evaluation by GC-MS. (n = 4 per group. Microglia from more than 10 brains were pooled for each sample. Both male and female mice were used.) Data are presented as mean values ± SEM. Differential metabolites were identified using a two-sided Student’s t-test.c. Heatmap for DEGs of cultured primary microglia from P0 Flox and ΔMFE-2 mice treated with brain extraction containing Aβ plaques from 8-month-old 5xFAD mice by bulk RNAseq. (n = 3 per sample. Microglia from more than 5 brains were pooled for each sample. Both male and female mice were used.) d. Flow cytometer analysis of inflammatory phenotype of primary microglia from 8-month-old Flox, ΔMFE-2, 5xFAD, and 5xFADΔMFE-2 mice after high-fat diet or control diet treatment. (n = 3 per each group, male) in Fig. 5f.
