Extended Data Fig. 7: CKBA targeting MFE-2 to resolve neuroinflammation.
a. Representative immunofluorescences result of MitoSOX stain in primary microglia treated with 100 μM AA (Arachidonic acid) combined with or without 5 μM CKBA. (n = 5 per each group). Scale bar, 100 μm. b. Timeline of the study and overview of the experimental design for vehicle or CKBA treatment in the 5xFAD mice experimental setup. c. LC-MS analysis of CKBA concentration in blood serum and brain tissue 12 hours after a single dose of CKBA administration at various dosages for wildtype mice. d. Results of Morris water maze assays of 1-year-old Vehicle-5xFAD, CKBA-5xFAD and CKBA-5xFADΔMFE-2. (n = 10 per each group. Both male and female mice were used.) Time in targeted quadrant and total swimming length were quantified. e. Representative immunofluorescences result of CD68 in sections from Vehicle-5xFAD, CKBA-5xFAD and CKBA-5xFADΔMFE-2 mice. (n = 4 per each sample, male). CD68 in green, microglia in red and nuclei in blue. Scale bar, 10 μm. Statistical analyses were performed by one-way ANOVA (a, c, d, e) and two-way ANOVA (d). All data were presented as mean values ± SEM. Specific p values are indicated in the figure.
