Extended Data Fig. 7: Restoration of Cdk3 premature mutation accelerates cell cycle reentry-induced neuronal death (CRND).
From: CDK3 induces neuronal death and brain atrophy in Alzheimer’s disease
(a-b) The number of genes per nucleus grouped by donor in panel a is as follows: WT: 9,791 nuclei, Cdk3(561DM): 10,797 nuclei, 5xFAD: 9,742 nuclei, 5xFAD;Cdk3(561DM): 9,550 nuclei, PS19: 9,960 nuclei, PS19;Cdk3(561DM): 11,456 nuclei. Additionally, the number of genes per neuronal nucleus grouped by donor in panel b is as follows: WT: 6,664 nuclei, Cdk3(561DM): 7,406 nuclei, 5xFAD: 6,227 nuclei, 5xFAD;Cdk3(561DM): 6,453 nuclei, PS19: 5,959 nuclei, PS19;Cdk3(561DM): 8,941 nuclei. The upper and lower bounds of each violin plot represent the maximum and minimum number of genes detected per nucleus, respectively. In the box plot, the upper and lower whiskers correspond to the upper and lower quartiles, while the hollow central point indicates the mean value. (c) Among the 22 neuronal sub-clusters analyzed for up-regulated genes, cluster 2 contains a total of 960 up-regulated genes. (d) A heatmap displaying the top marker genes for each cluster reveals that Ywhab, Fth1, Ywhah, Prnp, and Rps8 can be considered as marker genes for cluster 2. (e) Scatter plots depicting the expression intensity of marker genes associated with different types of cell death (including apoptosis, necroptosis, ferroptosis and pyroptosis) are presented based on sample source.
