Extended Data Fig. 8: Workflow of scHOB on human organoid-based single-cell multimodal data. | Nature Aging

Extended Data Fig. 8: Workflow of scHOB on human organoid-based single-cell multimodal data.

From: Integrating polygenic signals and single-cell multiomics identifies cell-type-specific regulomes critical for immune- and aging-related diseases

Extended Data Fig. 8

(1) Through systematic collection and curation, scHOB has gathered and curated over 1.5 million cells from 10 widely-adopted human organoids and their matching fetal tissues to establish an organoid single-cell multiomics database, including 83.4% scRNA-seq cells and 16.6% scATAC-seq cells. (2) The database queries encompass multiple lines of information, including gene expression, cell type, various protocols, chromatin accessibility, and motif activity. (3) scHOB features four main modules: the specificity module, comparison module, communication module, and gene-drug module. (4) Examples to demonstrate the utility of scHOB in gaining biological and therapeutic insights for human organoid development and disease etiology. For example, we applied scMORE to integrate human cerebral organoid single-cell multimodal data with GWAS summary datasets on eight psychiatric disorders for identifying psychiatric disorder-relevant eRegulons in given cellular contexts.

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