Extended Data Fig. 2: The scMORE method for identifying cell type-specific eRegulons associated with lymphocyte percent trait.
a. Scatter plot illustrating that scMORE identifies lymphocyte percent trait-relevant eRegulons specifically within CD8+ T cells; Red dots represent significant eRegulons, while gray dots indicate non-significant eRegulons. Significance was assessed by a Monte-Carlo permutation test (one-sided, upper tail; n = 1,000 iterations; no multiple correction). b. Scatter plot displaying that no significant eRegulon was identified by scMORE in monocyte cell type. Significance was assessed by a Monte-Carlo permutation test (one-sided, upper tail; n = 1,000 iterations; no multiple correction). c. UMAP showing single cell-level activity scores of three additional significant eRegulons that are significantly associated with lymphocyte percent trait. The regulon activity score for each eRegulon is calculated using the AddModuleScore function in Seurat. d. Empirical distribution of TRSs for seven eRegulons (FOXOP1, IKZF1, ZEB1, ZNF721, EST1, FOXP1, and LEF1) that were significantly or suggestively associated with the lymphocyte percent trait in CD8+ T cells. Significance was assessed by a Monte-Carlo permutation test (one-sided, upper tail; n = 1,000 iterations; no multiple correction). e. Empirical distribution of TRSs for the seven eRegulons showing significant or suggestive associations with the lymphocyte count trait in CD8+ T cells. Significance was assessed by a Monte-Carlo permutation test (one-sided, upper tail; n = 1,000 iterations; no multiple correction). Note: eRegulons were classified as follows: (1) Significant eRegulons: PGRS < 0.05, PCTS < 0.05, and PTRS < 0.05, (2) Suggestive eRegulons: PGRS < 0.05, PCTS < 0.1, and PTRS < 0.05, (3) Non-significant eRegulons: Do not meet the criteria for significant or suggestive eRegulons.
