Extended Data Fig. 5: Hippocampal Wdfy1 overexpression or downregulation alters bone phenotypes and the bone metabolism-regulatory role of Ser-EVs.
a, b, Femoral μCT images (a) and bone microstructural parameters (b) in AAV2-Con- or AAV2-Wdfy1-treated 4-month-old young female mice, and from AAV2-shCon- or AAV2-shWdfy1-treated 15-month-old male mice. H.I.P: hippocampal injection. Scale bar: 1 mm. n = 10 (young) or 7 (aged) per group. c, Three-point bending test of femoral ultimate load. n = 8 (young) or 7 (aged) per group. d, qRT-PCR for femoral Runx2 expression and ELISA for serum OCN. qRT-PCR: n = 9 (young) or 7 (aged) per group. ELISA: n = 9 (young: AAV2-Con-H.I.P), 8 (young: AAV2-Wdfy1-H.I.P), or 7 (aged: both groups). e, ORO staining images and quantification of ORO+ areas in distal femurs. Scale bar: 50 μm. n = 10 (young) or 7 (aged) per group. f, qRT-PCR for femoral Pparγ expression. n = 9 (young: AAV2-Con-H.I.P), 10 (young: AAV2-Wdfy1-H.I.P), or 7 (aged: both groups). g–i, qRT-PCR for femoral Runx2 (g) and Pparγ (h), and ELISA for serum OCN (g) and CTX-I (i) in vehicle- or WDFY1-treated 4-month-old young female mice by intramedullary injection. n = 9 (qRT-PCR for Runx2 in WDFY1 group) or 10 (others) per group. j–l, Femoral μCT images (j), bone microstructural parameters (k), and ultimate load (l) in 4-month-old young female mice treated with Ser-EVs from different groups. Scale bar: 1 mm. n = 10 per group. Data are mean ± SD. Statistics: unpaired, two-tailed Student’s t-test (b–i, k, and l).
