Extended Data Fig. 2: Activation of Piezo1 induces HSC proliferation and differentiation.
From: Shear stress governs hematopoietic stem cell fate to promote inflammation-induced aging
a,b, Representative flow cytometry plots of LT-HSCs, CD150high LT-HSCs (a), GMPs, and CMPs (b) and absolute cell numbers of these populations in the bone marrow of mice treated with Yoda1 (n = 5 mice per group; LT-HSCs: Yoda1 versus Vehicle P = 0.018; CD150high LT-HSCs: Yoda1 versus Vehicle P = 0.004; GMPs: Yoda1 versus Vehicle P = 0.008; CMPs: Yoda1 versus Vehicle P = 0.044). c, Cell counts of CLPs in bone marrow (n = 5 mice per group). d, Cell counts of neutrophils, Ly6Chi monocytes, B cells, and T cells in blood (n = 5 mice per group; neutrophils: Yoda1 versus Vehicle P = 0.015; Ly6Chi monocytes: Yoda1 versus Vehicle P = 0.016). e, Cell counts of RBCs in blood (n = 5 mice per group). f,g, Percentages of BrdU+ (f) and quiescent G0 (g) LT-HSCs (n = 5 mice per group; BrdU+ LT-HSCs: Yoda1 versus Vehicle P = 0.032; G0 LT-HSCs: Yoda1 versus Vehicle P = 0.045). h, CFU assay of equal numbers of LT-HSCs (n = 5 mice per group; CFU-GM/G/M: Yoda1 versus Vehicle P = 0.011). i, SiC division assay of LT-HSCs (n = 5 mice per group; divided cells: Yoda1 versus Vehicle P = 0.004). j, Apoptosis frequency of LT-HSCs (n = 5 mice per group). k, Ferroptosis susceptibility analysis of LT-HSCs (n = 5 mice per group). l, Cell counts of LT-HSCs in blood (n = 5 mice per group). m,n, Cell counts of LT-HSCs (m) and GMPs (n) in the bone marrow of Piezo1f/f;Vav mice treated with Yoda1 (n = 5 mice per group; LT-HSCs: Piezo1f/f;Vav+Yoda1 versus Piezo1f/f+Yoda1 P < 0.001; GMPs: Piezo1f/f;Vav+Yoda1 versus Piezo1f/f+Yoda1 P < 0.001). o, Cell counts of neutrophils and Ly6Chi monocytes in blood (n = 5 mice per group; neutrophils: Piezo1f/f;Vav+Yoda1 versus Piezo1f/f+Yoda1 P < 0.001; Ly6Chi monocytes: Piezo1f/f;Vav+Yoda1 versus Piezo1f/f+Yoda1 P < 0.001). p, Schematic view of the fetal liver from E16.5 mice. q, Cell counts of LT-HSCs, GMPs, and CLPs in fetal liver (n = 5 mice per group; LT-HSCs: Piezo1f/f;Vav versus Piezo1f/f P = 0.046; GMPs: Piezo1f/f;Vav versus Piezo1f/f P = 0.033). f/f: Piezo1f/f, f/f;Vav: Piezo1f/f;Vav. r, Percentages of BrdU+ LT-HSCs in fetal liver (n = 5 mice per group; Piezo1f/f;Vav versus Piezo1f/f P = 0.024). FL: fetal liver. s, CFU assay of equal numbers of fetal liver LT-HSCs (n = 5 mice per group; CFU-GM/G/M: Piezo1f/f;Vav versus Piezo1f/f P = 0.011). The data are representative of two (i) and three (a-h,j-o,q-s) independent experiments. Data are the mean value ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Statistical significance was determined by two-sided Student’s t test (a-l,q-s) or two-way ANOVA with Tukey’s post hoc test (m-o).
