Extended Data Fig. 7: Effects of metformin on cytosolic DNA/RNA sensing pathways.
From: Metformin inhibits nuclear egress of chromatin fragments in senescence and aging
a, IMR90 cells were cultured in 5 mM media with or without 5 mM metformin for 2 days. The cells were then transfected with ISD and were harvested 24 hours later, followed by RT-qPCR analyses with indicated genes. Results shown are mean values with s.d. normalized to those of Lamin A/C. Results are from three biological replicates. b, IMR90 cells were induced to senescence by IR. On day 8, the cells were cultured in 5 mM glucose media with or without 5 mM metformin. The cells were then harvested on day 14 and analyzed for cytosolic mitochondrial DNA using mitochondrial D-loop primer. See Methods for details. Results shown are mean values with s.d. P values were calculated with one-way ANOVA coupled with Tukey’s post hoc test. Results are from three biological replicates. c-d, IMR90 cells as indicated were stained by antibodies recognizing dsRNA (J2) and TOMM20. Scale bar: 10 μm. dsRNA signals outside of TOMM20 meshwork indicate cytosolic dsRNA, and were presented as mean values with s.d. (d). Each spot represents an individual cell. P values in this figure were calculated with one-way ANOVA coupled with Tukey’s post hoc test.
