Extended Data Fig. 8: ALIX undergoes autophagy-lysosome degradation upon glucose limitation or AMPK activation.
From: Metformin inhibits nuclear egress of chromatin fragments in senescence and aging
a, IMR90 cells were left untreated or cultured in 1 mM glucose media for 36 hours. The cells were then fixed, stained with antibodies as indicated, and imaged under a confocal microscopy. Scale bar: 5 μm. b, Related to Fig. 4d, additional images of Flag-ALIX and mutant in glucose-starved or compound 991 treatment conditions. Scale bars are labeled in images. c, IMR90 cells were glucose-starved for 3 days. MG132 was added for the last 2 days. The cell lysates were analyzed by immunoblotting. d, mCherry–GFP–ALIX was stably expressed in IMR90 cells. The cells were left untreated or glucose-starved for 1 day and were subjected to immunostaining and imaging under a confocal microscopy. Scale bar: 5 μm. Results shown in this figure are representative of at least three independent experiments.
