Extended Data Fig. 3: Roles of ESCRT-III in response to senescence and cytosolic dsDNA exposure.
From: Metformin inhibits nuclear egress of chromatin fragments in senescence and aging
a, IMR90 cells were treated with 50 μM etoposide for 48 hours, harvested on day 12, and subjected to imaging analyses. Results shown are mean values with s.d. from four randomly selected fields with over 200 cells. b, Etoposide-induced senescent IMR90 cells were analyzed by RT-qPCR, showing mean values with s.d., normalized to Lamin A/C. c and d, Primary BJ fibroblasts were induced to senescence with 20 Gy of IR and harvested on day 12, fixed, and subjected to imaging analyses. Bar graphs show mean values with s.d. from four randomly selected fields with over 200 cells. e, BJ cells were analyzed by RT-qPCR, normalized to Lamin A/C. Results shown are mean values with s.d. f and g, A549 cells were induced to senescence with 5 μM etoposide and harvested on day 14, fixed, and subjected to imaging analyses. Bar graphs show mean values with s.d. from three randomly selected fields with over 200 cells. h, A549 cells were analyzed by RT-qPCR, normalized to Lamin A/C. Results shown are mean values with s.d. i, IMR90 cells were stably infected with CRISPR constructs encoding sgControl, sgCHMP4B, or sgALIX. The cells were then transfected with ISD and were harvested 24 hours later, followed by RT-qPCR analyses with indicated genes. Results shown are mean values with s.d., normalized to those of Lamin A/C. Results in this figure are from at least three biological replicates. P values in this figure were calculated with one-way ANOVA coupled with Tukey’s post hoc test.
