Extended Data Fig. 6: Pharmacological inhibitors of LINE-1 reverse transcription or STING signaling exhibit no histological toxicity in major non-cardiac organs of aged mice.
From: Targeting age-related LINE-1 activation alleviates cardiac aging
a, Hematoxylin-eosin staining of liver, spleen, lung, kidney, and intestine from mice of 10 weeks old (10 wk), 48 weeks old (48 wk), 72 weeks old (72 wk), 72 weeks old and received 3TC treatment beginning at 48 weeks of age (72 wk + 3TC), and 72 weeks old and received H-151 treatment beginning at 48 weeks of age (72 wk + H-151). Scale bars, 200 μm. Histological injury was quantified as hepatic vacuolization area (%), splenic injury score, mean alveolar diameter, glomerular sclerosis index, and intestinal villus goblet-cell density. n = 6 mice per group. b, Measurement of serum levels of ALT (alanine aminotransferase), AST (aspartate aminotransferase), creatinine, and BUN (blood urea nitrogen). n = 5 mice per group. Data are presented as mean ± SEM with individual data points indicated. Groups were compared using one-way ANOVA and post hoc Dunnett′s test except for the splenic injury score that was analyzed with the Kruskal-Wallis test. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001.
