Extended Data Fig. 9: Pharmacological inhibitor of LINE-1 reverse transcription attenuates premature cardiac aging in Mov10-cKO mice.
From: Targeting age-related LINE-1 activation alleviates cardiac aging
a, Immunoblotting analysis of LINE-1 ORF1p and ORF2p protein levels in cardiomyocytes of Ctrl mice that received water (Ctrl + Water), Ctrl mice that received 3TC (Ctrl + 3TC), KO mice that received water (KO + Water), KO mice that received 3TC (KO + 3TC). GAPDH was used as a loading control. n = 4 mice per group. b, Immunoblotting analysis of p21 and p16 protein levels in the hearts from Ctrl mice that received water (Ctrl + Water), Ctrl mice that received 3TC (Ctrl + 3TC), KO mice that received water (KO + Water), KO mice that received 3TC (KO + 3TC). GAPDH was used as a loading control. n = 4 mice per group. c-d, Immunohistochemical analysis of p21 (c) and p16 (d) in mouse hearts from each group. Brown color in the nuclei indicates the cells positive for p21 and p16. Scale bar, 25 µm. n = 6 mice per group. e, SA-β-gal staining in mouse hearts from each group. Cyan color indicates cellular senescence. Scale bar, 50 μm. n = 6 mice per group. Data are presented as mean ± SEM with individual data points indicated. Groups were compared using two-way ANOVA post hoc Tukey test. ns, not significant, ***p < 0.001 compared to Ctrl + Water group; #p < 0.05, ###p < 0.001 compared to KO + Water group.
