Extended Data Fig. 3: Knockdown of LINE-1 alleviates senescence in Mov10-knockout H9C2 cells.
From: Targeting age-related LINE-1 activation alleviates cardiac aging
a, Immunoblotting analysis of LINE-1 ORF1p and ORF2p protein levels in wild-type (WT) and three independent lines of Mov10-knockout (KO-1, KO-2, and KO-3) H9C2 cells. GAPDH was used as a loading control. b, Quantification of LINE-1 ORF1p and ORF2p protein levels revealed by immunoblotting in WT and KO H9C2 cells transduced with Lenti-shCtrl or Lenti-shLINE-1. GAPDH was used as a loading control. n = 4 biological replicates per group. c, Immunoblotting analysis of p21 and p16 protein levels in WT and KO H9C2 cells transduced with Lenti-shCtrl or Lenti-shLINE-1. GAPDH was used as a loading control. n = 4 biological replicates per group. Data are presented as mean ± SEM with individual data points indicated. Groups were compared using two-way ANOVA post hoc Tukey test. **p < 0.01, ***p < 0.001 compared to WT H9C2 cells transduced with Lenti-shCtrl; ns, not significant, #p < 0.05, ##p < 0.01, ###p < 0.001 compared to corresponding WT or KO lines transduced with Lenti-shCtrl.
