Fig. 1: Characterization of ovarian health state and fecal microbial profiles of young and estropausal female mice.
From: Estropausal gut microbiota transplant improves measures of ovarian function in adult mice
a, Schematic diagram of the experimental setup of female aging cohorts. b, Boxplot of combined follicle count of young and estropausal mice (P value ~ 0.0065). c, Boxplot of serum concentrations of AMH (P value ~ 0.0079). d, Boxplot of ovarian health index of young and estropausal mice (P value ~ 0.0117). e, PCA result of CLR-transformed and batch-corrected ASV counts from young and estropausal female mice. Animals from four independent cohorts (C1–C4), n = 19–20 per group (variation due to animal death before experiment; n = 20 for young female mice; n = 19 for estropausal female mice). f, Boxplots of observed features (P value ~ 9.1 × 10−4) and Shannon entropy (P value ~ 9.7 × 10−6) indices of young and estropausal female mice. Medians from young female mice for each cohort were used to normalize indices per cohort. g, Differential abundance analysis results of microbial genera of young versus estropausal female mice using ALDEx2. h, Functional abundance prediction analysis of young versus estropausal female mice using PICRUSt2. i, Boxplot of β-glucuronidase activity from PICRUSt2 (P value ~ 0.3759). Medians from young female mice for each cohort were used to normalize indices per cohort. Boxplots show the median (center line), the 25th and 75th percentiles (bounds of the box), with whiskers extending to 1.5× the interquartile range (IQR). Individual datapoints are shown. Significance in nonparametric two-sided Wilcoxon rank-sum tests are reported for b–d,f,i; Benjamini–Hochberg-corrected P value of Wilcoxon tests are reported for g and h. C, cohort.
