Extended Data Fig. 5: Characterization of fecal microbial profiles of young and old male mice.
From: Estropausal gut microbiota transplant improves measures of ovarian function in adult mice
a, Schematic diagram of the experimental setup of male aging cohorts. b, Principal component analysis result of CLR-transformed and batch-corrected ASV counts from young and old male mice. Animals from three independent cohorts, n=14 per group (variation due to animal death or abnormal health issues prior to experiment). c, Principal coordinate analysis results of Bray–Curtis dissimilarity (top panel) and Jaccard (bottom panel) indices. d, Boxplots of observed features and Shannon entropy indices of young and old male mice (p values ~0.9633 and ~0.2456, respectively). Medians from young males for each cohort were used to normalize indices per cohort. e, Differential abundance analysis results of microbial genera of young and old male mice using ALDEx2. f, Functional abundance prediction analysis of young vs. old male mice using PICRUSt2. Boxplots show the median (center line), the 25th and 75th percentiles (bounds of the box), with whiskers extending to 1.5× the interquartile range (IQR). Individual datapoints are shown. Significance in nonparametric two-sided Wilcoxon rank-sum tests are reported for (d) and Benjamini–Hochberg corrected p value of Wilcoxon test are reported for (e) and (f). YM: Young male; OM: Old male.
