Fig. 5: Characterization of fecal microbial profiles of FMT recipient mice.
From: Estropausal gut microbiota transplant improves measures of ovarian function in adult mice
a, Schematic diagram of the experimental setup. b, PCA result of CLR-transformed and batch-corrected counts from young and estropausal female mice FMT recipient mice (FMT-YF and FMT-EF, respectively). c, Principal coordinate analysis results of Bray–Curtis dissimilarity (left) and Jaccard (right) indices. d, Boxplots of observed features (P value ~ 0.389) and Shannon entropy (P value ~ 0.818) indices of FMT-YF and FMT-EF mice. e, Volcano plot of differential abundance analysis results of microbial species of FMT-YF versus FMT-EF mice using ALDEx2. f, Bubble plot of top ten up- and downregulated microbial species in FMT-EF. g, Functional abundance analysis of FMT-YF versus FMT-EF mice using HUMAnN3 (q-value < 0.1). h, Boxplot of β-glucuronidase activity from HUMAnN3 (P value ~ 0.317). Median from FMT-YF group was used to normalize indices. Boxplots show the median (center line), the 25th and 75th percentiles (bounds of the box), with whiskers extending to 1.5× IQR. Individual datapoints are shown. Significance in nonparametric two-sided Wilcoxon rank-sum tests are reported for d and h, and Benjamini–Hochberg-corrected P values of Wilcoxon tests are reported for f.
