Research articles

The authors evaluate multiple factors to improve knock-in rates using CRISPR-mediated homology-directed repair in zebrafish, before demonstrating that prime editing is a superior alternative.

This Article presents the results of a global multi-laboratory study assessing the effects of housing conditions and experimental settings on novel tank test responses in adult zebrafish.

This study, which establishes reference intervals for biochemical and hematological parameters in healthy macaques and describes changes in these parameters after vaccination, will facilitate toxicity monitoring in preclinical vaccine studies.

This proof-of-concept study for the intranasal delivery of neuroactive drugs in adult zebrafish introduces a method that enables fast delivery of drugs such as arecoline and nicotine to the brain while requiring smaller quantities than water immersion methods.

This article describes a computational model to forecast population dynamics in a laboratory colony of common marmosets. The model can be used to predict future animal stock levels and support colony management.

The study shows that, compared with lean animals, obesity significantly impacts echocardiographic findings, infarct size, circulating biomarkers, mitochondrial respiratory capacity and mortality in a minipig model of myocardial infarction.

The authors used an implantable electrophysiological device to assess the effects of two commonly used anesthetics (isoflurane and pentobarbital) on supraventricular electrophysiology and arrhythmic substrate in male and female rats.

The authors compare muscle function, histology and molecular markers, and biochemical plasma profiles in male and female HSA^LR mice to identify potential sex differences in this mouse model of myotonic dystrophy type I.

Wound healing assays are commonly used to study tissue repair in mice. The authors show that wound localization and housing conditions are critical factors that can affect the reliability and reproducibility of the results of this assay.

This study explores the use of electrical stunning as a viable alternative to anesthetic overdose and rapid cooling for euthanizing zebrafish larvae, potentially improving the welfare of over 5 million zebrafish in biomedical research.

Differences in standard chow diets can influence mouse model phenotypes and impact experimental reproducibility. Here Knuth et al. show that the timing during which mice are exposed to standard chow can also affect phenotypic outcomes.

The marble burying task (MBT) is used to measure anxiety-like behavior, relying on a snapshot of the number of marbles buried at the end of the test. This Brief Communication introduces a more extensive behavioral analysis to improve the translational value of MBT for clinical psychological phenotypes.

This Protocol describes a minimally invasive technique for central nervous system drug delivery in rodents. The procedure allows the use of the transnasal route to deliver blood–brain barrier-impermeant drugs.

This study describes a new pig model of autism induced by embryonic exposure to valproic acid. The pig model displays behavioral alterations, abnormal gait and increased anxiety, which recapitulate key features of autism.

This systematic review identifies substantial heterogeneity in the experimental conditions in zebrafish nutrition studies, highlighting the urgent need for further research and the importance of standardized parameters for future zebrafish studies.

The authors describe a novel mouse model of cardiovascular–kidney–metabolic syndrome induced by unilateral nephrectomy and a Western diet rich in carbohydrates, fat and salt, which could be used to study human condition and identify therapies.

This Protocol describes a novel ischemic heart injury method in neonatal mice via cauterization of the root of the left coronary artery. The procedure is technically straightforward, requiring less than 10 min per mouse for completion.

Meijs et al. perform an electrophysiological investigation of cortical responses in a pig nerve injury model, showing the role of layer III–VI neurons in altered primary somatosensory cortex excitability after nerve injury.

Severe combined immunodeficiency (SCID) pigs do not live long enough to reproduce naturally. Monarch et al. show that reconstituting the immune system of SCID piglets with fetal bone allografts extends their lifespan, allowing them to reach puberty.

This study shows that using gene-editing technology in oocytes using Platinum TALEN is an effective method for producing genetically modified marmosets by exon skipping.