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Scalable fabrication of perovskite films for large-area solar cells remains a critical challenge. Jin et al. identify the solvent-precursor interaction time as a crucial, yet overlooked, kinetic parameter controlling crystallization and propose a protocol for manufacturing high-quality perovskite films.

Synthetic methylotrophic S. cerevisiae often faces energetic constrains during one-carbon assimilation. Here, the authors address this issue by engineering of heterologous methanol-formate-formaldehyde oxidation pathways to enable CO₂ assimilation via non-native Calvin cycle during methanol fermentation.

The residual methylammonium cation from additives compromises the operational stability of formamidinium lead iodide-based solar cells under thermal/light stress. Here, authors introduce MASCN to enhance film formation in ethyl acetate antisolvent and achieve efficiency of 26.1% for stable devices.

Multidrug efflux pumps actively expel a wide range of toxic substrates from bacteria and play a major role in drug resistance. Here authors show the in situ structure of the efflux pump AcrAB-TolC obtained by electron cryo-tomography and subtomogram averaging.

Assembly-line polyketide synthases perform modular enzymatic synthesis of medically important natural products. In this study, the authors used site-selective crosslinking to probe structural states that reveal how substrate carrier proteins interact with successive modules in a representative assembly line.

The 4Pi-SIM microscope combines the benefits of structured illumination microscopy with 4Pi geometry for improved axial resolution, offering isotropic, multicolor super-resolution imaging of dynamic processes in living cells.

The early stages of viral fusogen conformational change required for enveloped viruses to infect cells is unclear. Here, authors capture the herpesvirus fusogen, glycoprotein B, in early transitional states by cryo-EM.

In this work the authors investigate two types of secretins in Escherichia coli, GspD_α and GspD_β, and report the Cryo-ET in situ structures of their key intermediate states of during the translocation process. Yielding a resolution ranging from 9 Å to 19 Å, the structures allow the identification of different membrane interaction patterns and ways of transitioning the peptidoglycan layer. These results suggest two distinct models for the membrane translocation of GspD_α and GspD_β and provide insights into the inner to outer membrane biogenesis of T2SS secretins.

e2gmm uses a deep neural network with a Gaussian representation to resolve the compositional and conformational variability within biomolecules using cryo-EM data.

In this work, the authors combine cryo-electron tomography of vitrified virions, mass spectrometry, modeling, molecular dynamics and infectivity assay and report that a hinge glycan on viral spike protein influences virus infection and immune evasion.

ESM-Ezy combines the ESM-1b protein language model with similarity analysis to predict enzymatic functions in low-similarity sequences. It identifies high-performance biocatalysts, such as novel multicopper oxidases and L-asparaginases, with enhanced efficiency, stability, and industrial potential, advancing sustainable biotech solutions.

Here authors report cryo-EM structures of IP₃R1 which provide atomic details of IP₃, Ca²⁺ and ATP binding. Molecular motions of key domains and sidechains were found to regulate ligand binding and gating, which are validated by functional assays.

Cocrystal and cryo-EM structures of Geobacillus kaustophilus glyQ and Bacillus subtilis glyQS T-box-tRNA complexes establish a universal mechanism of amino acid sensing on tRNAs and gene regulation by T-box riboswitches.

An integrated pipeline for processing cryo-ET data implemented in EMAN2 streamlines data processing to minimize human bias, and improves the quality and resolution of resulting macromolecular structures, both in vitro and in cells.

Quantum error correction will be the key to allow large-scale quantum computing operations in the future. Here, the authors use a superconducting qubit system to demonstrate quantum error correction of a distance-three logical qubit in the heavy-hexagon subsystem code.

DNA supercoiling strongly affects its metabolism. By electron cryo-tomography, biochemical assays and molecular dynamics simulations, here the authors show that supercoiled DNA minicircles adopt unique and wide distributions of three-dimensional conformations, many with disrupted base pairs.

Fractal topologies are ubiquitous, and synthetic fractal objects provide unique functional advantages by virtue of their high surface area:volume ratios. Now, a computationally guided bottom-up design approach for constructing protein-based fractal assemblies in response to phosphorylation has been developed. Designed assemblies are shown to perform reversible and efficient molecular capture.

This manuscript describes a refinement protocol that extends the e2gmm method to optimize both the orientation and conformation estimation of particles to improve the alignment for flexible domains of proteins.

Machine learning based molecular model refinement algorithm generates series of molecular models from CryoEM datasets, which describe the dynamics of the macromolecular system and have near-perfect geometry scores.

The primary cilium is a microtubule-based organelle that functions in sensory and signal transduction; the authors demonstrate here that autophagic degradation of the oral-facial-digital syndrome 1 (OFD1) protein at centriolar satellites promotes primary cilium biogenesis, and that autophagy modulation might provide a novel means of ciliopathy treatment.

An algorithm and software tool automating the annotation of subcellular features in cryo-electron tomography data is presented.

Molecular and cellular characterization of callus formed from the rice scutellum reveals involvement of an embryo-like developmental program, in contrast to callus formed from the vasculature, which is regulated by genes involved in root organogenesis.