Single-molecule techniques can shed light on biochemical dynamics with high precision, but they require a significant photon number per frame and are hampered by the limited number of photons emitted by each fluorophore before photobleaching. Here, the authors introduce MUFFLE, a deep-learning denoising tool that enables single-molecule FRET with up to 10-fold reduction in photon requirement per frame.
- Yu Miao
- Yuxiao Cheng
- Chunlai Chen
