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This protocol describes how to design and build a centrifuge force microscope that is 3D printed to fit within an existing benchtop centrifuge and enables the simultaneous probing of hundreds to thousands of single-molecule interactions using tethered particles.
A protocol for the preparation of crystals for microcrystal electron diffraction provides X-ray crystallographers and cryo-electron microscopists access to a method that facilitates the determination of molecular structures from submicrometer crystals at atomic resolution.
We present a protocol for preparing microcrystal samples for cryo-EM diffraction imaging, including room temperature solid-state small molecules and soluble and membrane protein crystals.
Poorly performing antibodies contribute to the reproducibility crisis in biomedical research. The market offers millions of antibodies and validating them is a cumbersome process. Now, the non-profit Antibody Characterization through Open Science (YCharOS) organization shares a step-by-step protocol for rigorous antibody validation using genetic (knockout) cell line controls.
YCharOS is a consensus platform for antibody characterization developed through collaboration between academia and industry, enabling direct comparisons among antibodies that target a specific protein in three common applications.
A recent protocol for PASTE (programmable addition via site-specific targeting elements) combines prime editing and site-specific integrases to integrate large genetic payloads.
Programmable addition via site-specific targeting elements (PASTE) combines the specificity, efficiency and cargo size benefits of site-specific integrases with the programmability of prime editing for precise and efficient integration of large DNA sequences into mammalian genomes.
Standardized neuroimaging protocols are essential to advancing comparability and replicability. Now, a comprehensive magnetic resonance imaging acquisition and analysis protocol from the Rhineland Study provides a valuable framework for image acquisition and analysis that is suitable for both large- and small-scale neuroimaging studies.
A neuroimaging protocol presenting versatile high-resolution and time-efficient MRI acquisition and processing strategies suitable for large-scale and long-term population studies, which include structural, diffusion-weighted and functional MRI.
The members of Independent SAGE reflect on their experience in setting up, developing and running a science communication platform and service to the public during the COVID-19 pandemic
This deep learning platform enables large chemical spaces to be mined to identify substructures underlying predicted activity, with explainable reasoning behind the predictions. The protocol focuses on discovering structural classes of antibiotics but can be generally applied to other small molecules.
In this protocol, the tissue clearing process is carried out using a multiwell plate and insert, which allows the clearing steps to be conducted in parallel across multiple samples.
Generation and long-term culture of a human pluripotent stem cell-derived human cerebellar organoid model, which successfully replicates the cellular diversity of the fetal cerebellum along with some of its distinct cytoarchitectural features.
ChromEMT combines a fluorescent DNA binding dye that selectively enhances DNA and nucleosomes in electron microscopy with multi-tilt tomography, to enable the imaging and reconstruction of nuclear chromatin ultrastructure and 3D organization.
The facile synthesis of the 432 helicoid III nanoparticle morphology, displaying a Kuhn’s dissymmetry factor (g-factor) of 0.2, is detailed by using l-glutathione in an aqueous solution.
This protocol describes the optimization of RNA preparation conditions for cryo-EM structure determination, along with cryo-EM processing pipelines to resolve RNA dynamics and conformational changes, and workflows to generate moderate- to high-resolution cryo-EM density maps.
