Cytosine modifications within genomic DNA can serve as important epigenetic markers, but sequencing them at single-base resolution requires chemical modification-selective C-to-T code conversion with potential complex analysis. Now an unnatural base pair enables the sequencing of an epigenetic modification—5-formylcytosine—at single-base resolution within a Sanger sequencing format.
- David Schmidl
- Sidney M. Becker
- Shankar Balasubramanian
