MicroRNAs (miRNAs) are short RNAs that are ubiquitous, potent regulators of gene expression; however, as miRNA activity requires base pairing with only 6–8 nucleotides of messenger RNA, predicting target mRNAs is a major challenge. By using a method known as HITS-CLIP, combined with bioinformatic analysis, it has now been possible to demonstrate how the in vivo interactions between miRNAs and the mRNA targets can be validated.
- Sung Wook Chi
- Julie B. Zang
- Robert B. Darnell
